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Tg(Prnp-HTT*82Q)52Caro
Transgene Detail
Nomenclature
Symbol: Tg(Prnp-HTT*82Q)52Caro
Name: transgene insertion 52, Christopher A Ross
MGI ID: MGI:5304797
Synonyms: N586-82Q
Transgene: Tg(Prnp-HTT*82Q)52Caro  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6 x C3H)F1
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Prnp-HTT*82Q)52Caro expresses 1 gene
 
Mutation detailsThis transgene expresses the N-terminal 586 amino acids of human huntingtin (Htt) cDNA with a polyglutamine repeat length of 82, under the control of the mouse prion protein promoter. Amino acid 586 is the putative cleavage site of Htt by caspase 6 and so this transgene contains the caspase 6 fragment that would result from cleavage by caspase 6. This represents an intermediate-sized fragment. The mouse prion protein promoter drives transgene expression in the neurons of the central nervous system. Western blots show wide distribution of the protein in all brain regions tested. Immunoblots show that the N586-82Q fragment is cleaved into smaller fragments over time. Two lines were created, 52 and 91. Line 52 is a high-expressing line. (J:179364)
Phenotypes
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Disease models
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:179364 Waldron-Roby E, et al., Transgenic Mouse Model Expressing the Caspase 6 Fragment of Mutant Huntingtin. J Neurosci. 2012 Jan 4;32(1):183-193
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
02/25/2015
MGI 5.21
The Jackson Laboratory