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Hprttm87(Ple15-lacZ)Ems
Targeted Allele Detail
Nomenclature
Symbol: Hprttm87(Ple15-lacZ)Ems
Name: hypoxanthine guanine phosphoribosyl transferase; targeted mutation 87, Elizabeth M Simpson
MGI ID: MGI:5303443
Synonyms: NR2E1-lacZ
Gene: Hprt  Location: ChrX:52988137-53021659 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS4311, mEMS4319, mEMS4328, mEMS4333
Germline Transmission:  Earliest citation of germline transmission: J:183689
Parent Cell Line:  mEMS1202 (ES Cell)
Strain of Origin:  (B6.129P2-Hprtb-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion     Vector Type: Not Applicable
 
Mutation detailsThe Ple15-lacZ plasmid (pEMS1485) was created by inserting the Ple15 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human chromosome 8 open reading frame 46 (C8orf46) gene) into the multiple cloning site of the pEMS1313 plasmid vector backbone. This placed the Ple15 MiniPromoter upstream of an intron, a full F5 mutant-frt site, a Kozak consensus sequence, a beta-galactosidase (lacZ) gene, a full wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple15-lacZ construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells. (J:164356)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt Mutation:  1261 strains or lines available
References
Original:  J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
05/15/2018
MGI 6.12
The Jackson Laboratory