About   Help   FAQ
Hprt1tm87(Ple15-lacZ)Ems
Targeted Allele Detail
Summary
Symbol: Hprt1tm87(Ple15-lacZ)Ems
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 87, Elizabeth M Simpson
MGI ID: MGI:5303443
Synonyms: NR2E1-lacZ
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance: Hprt1tm87(Ple15-lacZ)Ems page
Mutation
origin
Mutant Cell Lines:  mEMS4311, mEMS4319, mEMS4328, mEMS4333
Germline Transmission:  Earliest citation of germline transmission: J:183689
Parent Cell Line:  mEMS1202 (ES Cell)
Strain of Origin:  (B6.129P2-Hprt1b-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion     Vector Type: Not Applicable
 
Mutation detailsThe Ple15-lacZ plasmid (pEMS1485) was created by inserting the Ple15 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human chromosome 8 open reading frame 46 (C8orf46) gene) into the multiple cloning site of the pEMS1313 plasmid vector backbone. This placed the Ple15 MiniPromoter upstream of an intron, a full F5 mutant-frt site, a Kozak consensus sequence, a beta-galactosidase (lacZ) gene, a full wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple15-lacZ construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells. (J:164356)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1272 strains or lines available
References
Original:  J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/12/2024
MGI 6.23
The Jackson Laboratory