Hprt1^{tm184(Ple126-EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm184(Ple126-EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 184, Elizabeth M Simpson
• MGI ID: MGI:5303267
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6

Mutation origin

• Mutant Cell Lines: mEMS3279, mEMS3302, mEMS3305, mEMS3308
• Germline Transmission: Unknown
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The RP11-406M16 bacterial artificial chromosome (BAC) was obtained and retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector backbone. Next, an enhanced green fluorescent protein (EGFP) and frt-flanked kanamycin cassette were inserted directly at the ATG of the human lactase (LCT) gene; this created the Ple126-EGFP BAC (bEMS75, LCT). The kanamycin cassette was transiently removed via FLP recombination; leaving the EGFP gene followed by a single frt site. The final modified BAC was used as the source for the Ple126-EGFP transgene. The Ple126-EGFP transgene was targeted as a single copy knock-in to the Hprt^b-m3 mutant locus on the X Chromosome via electroporation into mEMS1204 embryonic stem cells. (J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1280 strains or lines available

References

• Original: J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
• All: 1 reference(s)