Nkx2-1^{tm1.1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Nkx2-1^{tm1.1(cre/ERT2)Zjh}
• Name: NK2 homeobox 1; targeted mutation 1.1, Z Josh Huang
• MGI ID: MGI:5302534
• Synonyms: Nkx2.1-CreER
• Gene: Nkx2-1 Location: Chr12:56578741-56583570 bp, - strand Genetic Position: Chr12, 24.42 cM, cytoband C1-C3
• Alliance: Nkx2-1^{tm1.1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Nkx2-1^{tm1.1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nkx2-1 (MGI:108067)

• Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene , an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the NK2 homeobox 1 locus (Nkx2-1). This construct was electroporated into embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to remove the neo selection cassette. These Nkx2.1-CreER (or Nkx2.1-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for a few additional generations (and the FLPe transgene was removed). (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx2-1 (mouse) Summary of all recombinase alleles driven by Nkx2-1. Comparative matrix view of recombinase activities Alliance mouse Nkx2-1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nkx2-1 Mutation: 24 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 46 reference(s)