Rr15^tm1Wrk
Targeted Allele Detail

Summary

• Symbol: Rr15^tm1Wrk
• Name: regulatory region 15; targeted mutation 1, Wolf Reik
• MGI ID: MGI:5301263
• Gene: Rr15 Location: Chr7:142219273-142219468 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr15^tm1Wrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178292
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Insertion
• Mutation details: A floxed neomycin selection cassette was inserted in "differentially methylated region 1" (DMR1) upstream of Igf2. Insertion was in HpaII site 4 located in a Lrrfip1 (GCF2) binding consensus sequence. Properly targeted mice were crossed to mice carrying Tg(Pgk1-cre)1Lni to excise the selection cassette and leave behind a single loxP site that disrupts the GCF2 binding element. (J:178292)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr15 Mutation: 0 strains or lines available

References

• Original: J:178292 Eden S, et al., An upstream repressor element plays a role in Igf2 imprinting. EMBO J. 2001 Jul 2;20(13):3518-25
• All: 1 reference(s)