About   Help   FAQ
Targeted Allele Detail
Symbol: Ppargtm1.1(tTA)Jmgr
Name: peroxisome proliferator activated receptor gamma; targeted mutation 1.1, Jonathan M Graff
MGI ID: MGI:5300919
Synonyms: PPARgamma-tTA
Gene: Pparg  Location: Chr6:115337912-115467360 bp, + strand  Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
Germline Transmission:  Earliest citation of germline transmission: J:178521
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Transactivator)
Mutation:    Insertion
Mutation detailsA targeting construct was electroporated into ES cells and by homologous recombination, a tTA-Frt-neo-Frt (tTA = tetracycline transactivator) sequence was inserted into the second coding exon of Pparg, creating an in-frame fusion between the amino terminus of Pparg and tTA. Correctly targeted ES cells were used to generate mice. The neo resistance cassette was removed from the germline by crossing Pparg mutants with FLPe-expressing mice. (J:178521, J:212531)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pparg Mutation:  32 strains or lines available
ES cell line = ZX1, a mutant A17 line carrying an inducible cassette exchange allele at Hprt. The A17 ES cell line used to make the ZX1 cells is a mutant E14TG2A parental ES cell line carrying an inserted rtTA sequence at the Rosa26 locus.
Original:  J:178521 Tang W, et al., White fat progenitor cells reside in the adipose vasculature. Science. 2008 Oct 24;322(5901):583-6
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.17
The Jackson Laboratory