Gt(ROSA)26Sor^{tm3(tetO-EGFP,-RNAi:T)Bgh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(tetO-EGFP,-RNAi:T)Bgh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Bernhard G Herrmann
• MGI ID: MGI:5297100
• Synonyms: KD3-T
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(tetO-EGFP,-RNAi:T)Bgh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:190628
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3(tetO-EGFP,-RNAi:T)Bgh} involves 1 genes/genome features (T) View all
• Mutation details: Recombinase mediated cassette exchange was used to insert a tetO, CAG regulated EGFP followed by a short hairpin RNAi specific for the Brachyury (T) gene followed by a PGK promoter into the Gt(ROSA)26Sor^tm1Bgh allele. The tetracycline silencer prevents expression of both the reporter and the shRNAi until mice are treated with doxycycline. (J:173434)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

Notes

ES cell lines = C57BL/6 and G4.

References

• Original: J:173434 Vidigal JA, et al., An inducible RNA interference system for the functional dissection of mouse embryogenesis. Nucleic Acids Res. 2010 Jun 1;38(11):e122
• All: 2 reference(s)