Tg(CD4-Tcra/EGFP,Tcrb)#Mfk
Transgene Detail

Summary

• Symbol: Tg(CD4-Tcra/EGFP,Tcrb)#Mfk
• Name: transgene insertion, Matthew F Krummel
• MGI ID: MGI:5296834
• Synonyms: OT-I-GFP Tg
• Transgene: Tg(CD4-Tcra/EGFP,Tcrb)#Mfk Location: unknown
• Alliance: Tg(CD4-Tcra/EGFP,Tcrb)#Mfk page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD4-Tcra/EGFP,Tcrb)#Mfk expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: The original OT-I transgenic constructs were obtained from F. Carbone (University of Melbourne, Melbourne, Victoria, Australia). The OT-Ialpha chain was tagged on the C terminus with a 20-aa flexible linker and a single enhanced GFP using standard molecular cloning techniques and was expressed under the CD4 enhancer and promoter without the silencer. The OT-Ibeta chain was expressed under its endogenous promoter. (J:177978)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:177978 Friedman RS, et al., Real-time analysis of T cell receptors in naive cells in vitro and in vivo reveals flexibility in synapse and signaling dynamics. J Exp Med. 2010 Nov 22;207(12):2733-49
• All: 1 reference(s)