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Tg(CD4-Tcra/EGFP,Tcrb)#Mfk
Transgene Detail
Summary
Symbol: Tg(CD4-Tcra/EGFP,Tcrb)#Mfk
Name: transgene insertion, Matthew F Krummel
MGI ID: MGI:5296834
Synonyms: OT-I-GFP Tg
Transgene: Tg(CD4-Tcra/EGFP,Tcrb)#Mfk  Location: unknown  
Alliance: Tg(CD4-Tcra/EGFP,Tcrb)#Mfk page
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(CD4-Tcra/EGFP,Tcrb)#Mfk expresses 2 genes
 
Mutation detailsThe original OT-I transgenic constructs were obtained from F. Carbone (University of Melbourne, Melbourne, Victoria, Australia). The OT-Ialpha chain was tagged on the C terminus with a 20-aa flexible linker and a single enhanced GFP using standard molecular cloning techniques and was expressed under the CD4 enhancer and promoter without the silencer. The OT-Ibeta chain was expressed under its endogenous promoter. (J:177978)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
References
Original:  J:177978 Friedman RS, et al., Real-time analysis of T cell receptors in naive cells in vitro and in vivo reveals flexibility in synapse and signaling dynamics. J Exp Med. 2010 Nov 22;207(12):2733-49
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory