Tg(tetO-CHRM3*)1Blr
Transgene Detail

Summary

• Symbol: Tg(tetO-CHRM3*)1Blr
• Name: transgene insertion 1, Bryan L Roth
• MGI ID: MGI:5296366
• Synonyms: tetO-hM3Dq, TRE-HA-hM3Dq, TRE-hM3Dq
• Transgene: Tg(tetO-CHRM3*)1Blr Location: unknown
• Alliance: Tg(tetO-CHRM3*)1Blr page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-CHRM3*)1Blr expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CHRM3 (1131)

  Chrm3

  (MGI:88398)

• Mutation details: The Tet-responsive P promoter which consists of seven direct repeats of the 36-bp sequence each containing the 19-bp tet operator sequence (tetO) is just upstream of a minimal human cytomegalovirus (CMV) promoter. This is upstream of a hemagglutinin epitope tag and a Gq-coupled human M3 muscarinic receptor. The wild-type human muscarinic 3 receptor sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y149C and A239G) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:154952)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:154952 Alexander GM, et al., Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors. Neuron. 2009 Jul 16;63(1):27-39
• All: 10 reference(s)