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Transgene Detail
Symbol: Tg(tetO-CHRM3*)1Blr
Name: transgene insertion 1, Bryan Roth
MGI ID: MGI:5296366
Synonyms: tetO-hM3Dq, TRE-HA-hM3Dq, TRE-hM3Dq
Transgene: Tg(tetO-CHRM3*)1Blr  Location: unknown  
Strain of Origin:  (C57BL/6 x SJL)F1
Transgene Type:    Transgenic (Inducible, Inserted expressed sequence)
Mutation:    Insertion
Tg(tetO-CHRM3*)1Blr expresses 1 gene
Mutation detailsThe Tet-responsive P promoter which consists of seven direct repeats of 36-bp sequence each containing the 19-bp tet operator sequence (tetO) is just upstream of a minimal human cytomegalovirus (CMV) promoter. This is upstream of a hemagglutinin epitop tag and a Gq-coupled human M3 muscarinic receptor. The wildtype human muscarinic 3 receptor sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y149C and A239G) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:154952)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:154952 Alexander GM, et al., Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors. Neuron. 2009 Jul 16;63(1):27-39
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
The Jackson Laboratory