Tg(Vav1-NPM1/ALK)1Sudt
Transgene Detail

Summary

• Symbol: Tg(Vav1-NPM1/ALK)1Sudt
• Name: transgene insertion 1, Suzanne D Turner
• MGI ID: MGI:5292256
• Synonyms: NPM-ALK Line 1, Tg(Vav-NPM/ALK)1Sudt, Vav/NPM-ALK Line 1
• Transgene: Tg(Vav1-NPM1/ALK)1Sudt Location: unknown
• Alliance: Tg(Vav1-NPM1/ALK)1Sudt page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Vav1-NPM1/ALK)1Sudt expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ALK (238)

  Alk	(MGI:103305)

  Transgene codes for a fusion protein of NPM1 and ALK.

  human

  NPM1 (4869)

  Npm1	(MGI:106184)

  Transgene codes for a fusion protein of NPM1 and ALK.

• Mutation details: This transgene contains the regulatory sequences of the mouse vav 1 oncogene (Vav), which is expressed ubiquitously and exclusively in hematopoietic cells, driving expression of the human tumor-associated NPM1/ALK fusion protein. This chimeric tyrosine kinase, comprising the N-terminal 40% of the ubiquitously expressed protein nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1) joined to the entire cytoplasmic tail of anaplastic lymphoma kinase (ALK), is expressed in tumors from a fusion gene resulting from the t(2;5)(p23;q35) chromosomal translocation that is present in 40-50% of anaplastic large cell lymphomas. Line 1 bears a single copy of the transgene, as estimated by Southern blot analysis. RT-PCR analysis of mRNA and immunoblot analysis of tissue lysates demonstrated the presence of transgene-derived transcripts and protein, respectively, in tumors, but not in normal thymus or spleen, of transgenic mice. (J:86490)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

This is one of two lines bearing this transgenic construct that develop tumors; the other was designated Line 3.

References

• Original: J:86490 Turner SD, et al., Vav-promoter regulated oncogenic fusion protein NPM-ALK in transgenic mice causes B-cell lymphomas with hyperactive Jun kinase. Oncogene. 2003 Oct 30;22(49):7750-61
• All: 3 reference(s)