Sst^{tm1.2(tTA2)Hze}
Targeted Allele Detail

Summary

• Symbol: Sst^{tm1.2(tTA2)Hze}
• Name: somatostatin; targeted mutation 1.2, Hongkui Zeng
• MGI ID: MGI:5292254
• Synonyms: SST-tTA2-D, SST-tTA2-DELTA, SST-tTA2-deltaNEO
• Gene: Sst Location: Chr16:23708323-23709708 bp, - strand Genetic Position: Chr16, 15.0 cM, cytoband cen-C3
• Alliance: Sst^{tm1.2(tTA2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176570
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Transactivator)
• Mutation: Insertion
• Mutation details: A targeting vector used to insert a synthetic modified tetracycline-regulated transactivator (tTA2S) gene and 3' UTR region of the somatostatin exon 2 into the ATG transcriptional start site of the somatostatin gene (Sst). Specifically, the targeting vector was designed with (from 5' to 3') an frt3 site, a Cre recombinase gene, a bovine growth hormone polyA sequence, a second frt3 site, a tTA2S gene, the 3' UTR region from somatostatin exon 2, an AttB site, PGK-Neo-polyA cassette, an frt5 site, an RNA splice acceptor, the 3' portion of the hygromycin gene with SV40 polyA signal, and an AttP site. (J:176570)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sst Mutation: 35 strains or lines available

References

• Original: J:176570 Zeng H, Direct Data Submission 2011/10/17. MGI Direct Data Submission. 2011
• All: 1 reference(s)