Fam219a^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Fam219a^tm1(KOMP)Mbp
• Name: family with sequence similarity 219, member A; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:5287740
• Gene: Fam219a Location: Chr4:41517691-41569538 bp, - strand Genetic Position: Chr4, 21.71 cM, cytoband B1
• IMPC: Fam219a gene page

Mutation origin

• Mutant Cell Lines: DEPD00574_3_B05, DEPD00574_3_B06, DEPD00574_3_B07, DEPD00574_3_B08, DEPD00574_3_C05, DEPD00574_3_C06, DEPD00574_3_C07, DEPD00574_3_C08, DEPD00574_3_D05, DEPD00574_3_D06, DEPD00574_3_D08, DEPD00574_3_E05, DEPD00574_3_F06, DEPD00574_3_F07, DEPD00574_3_G07, DEPD00574_3_G08, DEPD00574_3_H06, DEPD00574_3_H07, DEPD00574_3_H08
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 3648 starting at position 41520834 and ending at position 41520834 of Chromosome 4 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Fam219a Mutation: 14 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 1 reference(s)