Hoxb1^tm1.1Mist
Targeted Allele Detail

Summary

• Symbol: Hoxb1^tm1.1Mist
• Name: homeobox B1; targeted mutation 1.1, Michele Studer
• MGI ID: MGI:5287678
• Synonyms: Hoxb1^null, Hoxb1 null
• Gene: Hoxb1 Location: Chr11:96256578-96259082 bp, + strand Genetic Position: Chr11, 59.86 cM
• Alliance: Hoxb1^tm1.1Mist page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into the Hoxb1 locus upstream of the r4 enhancer (Rr140). A loxP-flanked neo cassette was inserted into intron 1. Internal to the first two loxP sites, two heterospecific FRT sites were also inserted such that by recombinase-mediated cassette exchange, exogenous sequences could be knocked into Hoxb1. Electroporation of a cre-expressing plasmid into an ES cell clone containing the modified locus resulted in excision of the regions between the different combinations of loxP sites. Deletion of the region between the proximal and distal loxP sites produced the Hoxb1^null allele. (J:197162)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hoxb1 Mutation: 24 strains or lines available

References

• Original: J:197162 Di Bonito M, et al., Assembly of the auditory circuitry by a Hox genetic network in the mouse brainstem. PLoS Genet. 2013;9(2):e1003249
• All: 2 reference(s)