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Dhx29TgTn(sb-cHS4,Tyr)2354AOve
Transgenic Allele Detail
Nomenclature
Symbol: Dhx29TgTn(sb-cHS4,Tyr)2354AOve
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 29; transgenic transposon lentiviral insertion 2354A, Paul A Overbeek
MGI ID: MGI:5287023
Synonyms: OVE2354A, OVE#2354A
Gene: Dhx29  Location: Chr13:113063988-113105966 bp, + strand  Genetic Position: Chr13, 63.93 cM
Transgene
origin
Strain of Origin:  FVB/N
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Transposon insertion
 
Mutation detailsThe SB-cHS4core-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2229) was designed with a Sleeping Beauty (SB) transposon (inward-facing SB arms flanking a tandem pair of cHS4 core insulator elements), followed by a mouse tyrosinase minigene (Tyro) and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The SB transposon and Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The SB transposon used in this transgene has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site), two copies of the 0.25 kb chicken beta-globin core insulator element (cHS4 core), and a second IR/DR sequence. The IR/DR sequences are inward-facing (pointed towards the cHS4core). The cHS4 core insulator element functions to terminate transcription of the upstream locus into which it integrates (and also enhance expression from the downstream Tyro minigene). The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. In line OVE2354F, a single copy of the lentiposon inserted into intron 26 [NCBI37/mm9; 5'-113,758,215(+)] in the antisense orientation. (J:175597)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dhx29 Mutation:  52 strains or lines available
References
Original:  J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory