Slc6a9^tm1.1Caz
Targeted Allele Detail

Summary

• Symbol: Slc6a9^tm1.1Caz
• Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 9; targeted mutation 1.1, Chiara Cazzin
• MGI ID: MGI:5085299
• Synonyms: Glyt1 lox
• Gene: Slc6a9 Location: Chr4:117692240-117726502 bp, + strand Genetic Position: Chr4, 53.62 cM
• Alliance: Slc6a9^tm1.1Caz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:168155
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site within intron 2 and the neomycin cassette flanked by Frt sites and an additional loxP site within intron 3 are inserted by homologous recombination. The conditional allele was generated by breeding with a germline Flp deleter strain Gt(ROSA)26Sor to delete the Neo cassette leaving a single loxP and Frt sites in intron 3. Although the cre-mediated deletion is predicted to result in the production of an in-frame transcript, the gene product from this allele is predicted to be non functional. (J:168155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc6a9 Mutation: 24 strains or lines available

References

• Original: J:168155 Cazzin C, et al., Adenoviral-mediated Cre expression effectively suppresses GlyT1 binding in the thalamic area of GlyT1 conditional knock-out mice. J Neurosci Methods. 2010 Nov 30;193(2):254-63
• All: 1 reference(s)