Efnb2^tm3.1Henk
Targeted Allele Detail

Summary

• Symbol: Efnb2^tm3.1Henk
• Name: ephrin B2; targeted mutation 3.1, Mark Henkemeyer
• MGI ID: MGI:5085271
• Synonyms: ephrin-B2^6YFdeltaV
• Gene: Efnb2 Location: Chr8:8667434-8711242 bp, - strand Genetic Position: Chr8, 3.42 cM
• Alliance: Efnb2^tm3.1Henk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173636
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion, Nucleotide substitutions
• Mutation details: The 6 tyrosines in the cytoplasmic domain were mutated to phenylalanine to prevent tyrosine phosphorylation, the GTC codon for valine in exon 5 was deleted, and a floxed neo cassette was inserted via homologous recombination. Cre mediated recombination removed the neo cassette. (J:173636, J:176056)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Efnb2 Mutation: 28 strains or lines available

References

• Original: J:173636 Dravis C, et al., Ephrin-B reverse signaling controls septation events at the embryonic midline through separate tyrosine phosphorylation-independent signaling avenues. Dev Biol. 2011 Jul 1;355(1):138-51
• All: 7 reference(s)