Mirc8^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc8^tm1Wtsi
• Name: microRNA cluster 8, including Mirlet7d, Mirlet7f-1, and Mirlet7a-1; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5050749
• Synonyms: Mirlet7a-1/Mirlet7f-1 cluster 13N1, Mirlet7f-1^{tm1(mirKO)Wtsi}
• Gene: Mirc8 Location: Chr13:48689488-48691748 bp Genetic Position: Chr13, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_8G7, mirKO_ES_PuDtk_8G8, mirKO_ES_PuDtk_8G9, mirKO_ES_PuDtk_8G10, mirKO_ES_PuDtk_8G12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: Mirlet7f-1, Mirlet7d and Mirlet7a-1 in this microRNA cluster were replaced via homologous recombination by a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. The deletion starts at position 48538339 and ends at position 48537810 of Chromosome 13 (Genome Build 38). (J:94077, J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc8 Mutation: 3 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)