Mirc6^tm2Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc6^tm2Wtsi
• Name: microRNA cluster 6, including Mir379 through Mir544; targeted mutation 2, Wellcome Trust Sanger Institute
• MGI ID: MGI:5050744
• Synonyms: Mir376c/Mir376a cluster 12N6, Mir376c^{tm1(mirKO)Wtsi}
• Gene: Mirc6 Location: Chr12:109675494-109695836 bp Genetic Position: Chr12, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_8F7, mirKO_ES_PuDtk_8F8, mirKO_ES_PuDtk_8F9, mirKO_ES_PuDtk_8F10
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: Mir376c, Mir654, Mir376b and Mir376a in this microRNA cluster were replaced via homologous recombination by a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc6 Mutation: 3 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)