Mirc4^tm2Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc4^tm2Wtsi
• Name: microRNA cluster 4, including Mir429 through Mir200b; targeted mutation 2, Wellcome Trust Sanger Institute
• MGI ID: MGI:5050735
• Synonyms: Mir200a/Mir200b cluster 4N1, Mir200a^{tm1(mirKO)Wtsi}
• Gene: Mirc4 Location: unknown Genetic Position: Chr4, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_5H7, mirKO_ES_PuDtk_5H8, mirKO_ES_PuDtk_5H9, mirKO_ES_PuDtk_5H10, mirKO_ES_PuDtk_5H11, mirKO_ES_PuDtk_5H12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: Mir200a, and Mir200b in this microRNA cluster were replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by LoxP and F3/FRT sites. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc4 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)