Otof^{tm2.1(cre/ERT2)Mull}
Targeted Allele Detail

Summary

• Symbol: Otof^{tm2.1(cre/ERT2)Mull}
• Name: otoferlin; targeted mutation 2.1, Ulrich Mueller
• MGI ID: MGI:5014097
• Synonyms: Otof-CreERT2
• Gene: Otof Location: Chr5:30524406-30619276 bp, - strand Genetic Position: Chr5, 16.48 cM, cytoband B1
• Alliance: Otof^{tm2.1(cre/ERT2)Mull} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Otof^{tm2.1(cre/ERT2)Mull} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Otof (MGI:1891247)

• Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an FRT-flanked PGK-Neo cassette into the initiation codon of the otoferlin (Otof) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice to establish the colony. Next, mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background to remove the FRT-flanked PGK-Neo cassette. The resulting Otof-CreERT2 mice were then bred to C57BL/6J inbred mice for approximately two generations (selecting away from the FLPe transgene). (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Otof (mouse) Summary of all recombinase alleles driven by Otof. Comparative matrix view of recombinase activities Alliance mouse Otof gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Otof Mutation: 108 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 4 reference(s)