Tg(Eno2-Prnp)#Aag
Transgene Detail

Summary

• Symbol: Tg(Eno2-Prnp)#Aag
• Name: transgene insertion, Adriano Aguzzi
• MGI ID: MGI:5013941
• Synonyms: NSE-PrP
• Transgene: Tg(Eno2-Prnp)#Aag Location: unknown
• Alliance: Tg(Eno2-Prnp)#Aag page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Eno2-Prnp)#Aag expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: Mice bearing this transgene express the normal, cellular mouse prion protein under control of the promoter of the enolase 2, gamma neuronal gene (Eno2). Of the prion protein gene (Prnp), it contains the first exon, first intron, and second exon followed immediately by the third exon, which contains the entire Prnp open reading frame (ORF), and the 3' untranslated region. This construct was inserted between the first intron and the second exon of the enolase 2 gene in a construct containing 2.8 kb of 5' flanking DNA, exon1, intron 1, and exon 2 (followed by the SV40 polyadenylation signal). Mice hemizygous for this transgene on a background lacking a functional endogenous prion protein gene express the transgene in neurons, including cerebellar granule cells (CGC), at higher levels than observed for the endogenous gene in wild-type mice. (J:98738)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:98738 Radovanovic I, et al., Truncated prion protein and Doppel are myelinotoxic in the absence of oligodendrocytic PrPC. J Neurosci. 2005 May 11;25(19):4879-88
• All: 2 reference(s)