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Pigftm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Pigftm1a(KOMP)Wtsi
Name: phosphatidylinositol glycan anchor biosynthesis, class F; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:5013736
Gene: Pigf  Location: Chr17:86997256-87025406 bp, - strand  Genetic Position: Chr17, 56.9 cM, cytoband E4-E5
Mutation
origin
Mutant Cell Lines:  EPD0805_3_A10, EPD0805_3_A11, EPD0805_3_A12, EPD0805_3_B11, EPD0805_3_B12, EPD0805_3_C09, EPD0805_3_C10, EPD0805_3_C11, EPD0805_3_C12, EPD0805_3_D11, EPD0805_3_E09, EPD0805_3_E10, EPD0805_3_E11, EPD0805_3_E12, EPD0805_3_F09, EPD0805_3_F10, EPD0805_3_F11, EPD0805_3_F12, EPD0805_3_G09, EPD0805_3_G10, EPD0805_3_G11, EPD0805_3_H09, EPD0805_3_H10, EPD0805_3_H11, EPD0805_3_H12
Germline Transmission:  Earliest citation of germline transmission: J:82809
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 87022400 of Chromosome 17 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87021607. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 2 lines available
Carrying any Pigf Mutation:  13 strains or lines available
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory