Tg(Rho-tTA)5Vmrg
Transgene Detail

Summary

• Symbol: Tg(Rho-tTA)5Vmrg
• Name: transgene insertion 5, Valeria Marigo
• MGI ID: MGI:5008627
• Synonyms: RhotTA1-L5, Tg(Rho-tTA)L5
• Transgene: Tg(Rho-tTA)5Vmrg Location: unknown
• Alliance: Tg(Rho-tTA)5Vmrg page

Transgene origin

• Strain of Origin: Crl:CD-1(ICR)

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: The transgene contains ~4.4 kb of the mouse rhodopsin gene promoter immediately upstream of the initiating ATG, which has been replaced by that of the modified tetracycline transactivator tTA1 - which has lower toxicity than and >2-fold the activity of tTA - followed by the SV40 polyadenylation signal. RT-PCR of the retinas of transgenic mice reveals expression of tTA beginning on postnatal day (P)8, which increases over several days and and is maintained thereafter. In situ hybridization localizes expression to the photoreceptor cells of the outer nuclear layer of the retina. Mice bearing both this and a tetracyline operator (tetO) driven luciferase reporter transgene express luciferase from P8 in the same cells, reaching a plateau by P13. Luciferase assays of other tissues of these mice demonstrate that induction is specific to the retinal outer nuclear layer. Expression of luciferase, but not of tTA1, is abolished within 24 h by doxycycline (Dox) exposure via drinking water and remains so for at least 10 d with continued exposure; luciferase is again detectable 4 days after cessation of treatment. (J:172631, J:172632)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two transgenic lines, designated RhotTA1-L5 and RhotTA1-L32, were derived from a single founder mouse with two independent integrations comprising different numbers of copies, L32 having the higher copy number by Southern blot analysis. Expression is more variable and induction of a reporter transgene occurs with slower kinetics and a lower maximum in L32 than in L5 mice; the kinetics of repression of reporter expression by Dox treatment also are slower in L32. (J:172631)

References

• Original: J:172631 Angeletti B, et al., An in vivo doxycycline-controlled expression system for functional studies of the retina. Invest Ophthalmol Vis Sci. 2003 Feb;44(2):755-60
• All: 2 reference(s)