Tg(Gjc1/EGFP)L43Kwi
Transgene Detail

Summary

• Symbol: Tg(Gjc1/EGFP)L43Kwi
• Name: transgene insertion L43, Klaus Willecke
• MGI ID: MGI:5008304
• Synonyms: Cx45eGFP-BAC, Tg(Gjc1EGFP)L43Kwi
• Transgene: Tg(Gjc1/EGFP)L43Kwi Location: unknown
• Alliance: Tg(Gjc1/EGFP)L43Kwi page

Transgene origin

• Strain of Origin: C57BL/6 x FVB/N

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This transgene is derived from the ~260 kb genomic DNA insert of a bacterial artificial chromosome (BAC) clone that contains the coding region and upstream and downstream regulatory elements of the mouse gap junction protein, gamma 1 gene (Gjc1, a.k.a. Cx45). The Gjc1 termination codon has been deleted and its coding sequence joined, in-frame, to the cDNA encoding enhanced green fluorescent protein (EGFP). Southern blot analysis shows that this line (L43) has integrated multiple copies of the transgenic construct. RT-PCR analysis detects the fusion transcript in brain, heart and lung, and immunoblot analysis detects the fusion protein in all three organs at about 2.7-fold the level of the endogenous protein and in similar relative amounts. Immunofluorescent imaging using anti-EGFP and anti-Cx45 polyclonal antibodies demonstrates subcellular co-localization of the fusion and endogenous GJC1/CX45 proteins. Immunoflourescence analysis of fixed tissue sections with anti-EGFP antibodies confirmed and expanded previous expression data for the endogenous gene and demonstrated expression in the atrial and ventricular working myocardium. (J:172341)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two transgenic mouse lines, designated L18 and L43, were analyzed; Southern blot analysis shows that line L18 has integrated a single copy and L43 multiple copies of the transgene construct. Expression of the fusion protien is higher in L43. The authors report "The transgenic offspring did not seem to be affected by the integration of the Cx45eGFP-BAC, e.g. showed the same properties whether it was derived from L18 or L43." The line(s) used for immunofluorescence analysis is/are not specified. (J:172341)

References

• Original: J:172341 von Maltzahn J, et al., C-terminal tagging with eGFP yields new insights into expression of connexin45 but prevents rescue of embryonic lethal connexin45-deficient mice. Eur J Cell Biol. 2009 Sep;88(9):481-94
• All: 2 reference(s)