Mcpt8^tm1(cre)Lky
Targeted Allele Detail

Summary

• Symbol: Mcpt8^tm1(cre)Lky
• Name: mast cell protease 8; targeted mutation 1, Richard M Locksley
• MGI ID: MGI:5007937
• Synonyms: Basoph8, Mcpt8^tm1(cre)Lksy, Mcpt8^YFP-Cre
• Gene: Mcpt8 Location: Chr14:56319623-56322730 bp, - strand Genetic Position: Chr14, 28.19 cM
• Alliance: Mcpt8^tm1(cre)Lky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:172122
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Mcpt8^tm1(cre)Lky expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mcpt8 (MGI:1261780)

• Mutation details: A 4.8 kb reporter cassette containing YFP, IRES site, humanized cre recombinase, bGH polyA, and a loxP-flanked neo selection marker was inserted at the Mcpt8 start site via homologous recombination in ES cells contain PrmCre (PC3 cells with Tg(Prm-cre)70Og). Upon crossing male chimeras to wild-type females, the loxP-flanked neo was deleted from the male germline by cre recombination, producing the final knock-in allele. (J:172122)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mcpt8 (mouse) Summary of all recombinase alleles driven by Mcpt8. Alliance mouse Mcpt8 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mcpt8 Mutation: 23 strains or lines available

References

• Original: J:172122 Sullivan BM, et al., Genetic analysis of basophil function in vivo. Nat Immunol. 2011 Jun;12(6):527-35
• All: 14 reference(s)