Mia3^tm1Lex
Targeted Allele Detail

Summary

• Symbol: Mia3^tm1Lex
• Name: MIA SH3 domain ER export factor 3; targeted mutation 1, Lexicon Pharmaceuticals
• MGI ID: MGI:5007248
• Gene: Mia3 Location: Chr1:183107682-183150894 bp, - strand Genetic Position: Chr1, Syntenic
• Alliance: Mia3^tm1Lex page

Mia3^tm1Lex/Mia3^tm1Lex embryos are dwarfed, edemic, and fail to form a mineralized skeleton

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:161922
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: A lacZ/neo fusion cassette was inserted in frame 11 bases into the beginning of the second exon, replacing this exon and all of exon3. Western blot analysis confirmed the absence of the large SH3 domain containing isoform and the presence of an about 80-90 kD isoform lacking the SH3 domain in homozygous embryos. (J:161922, J:171883, J:172737)

Generation of the Mia3^tm1Lex allele

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mia3 Mutation: 23 strains or lines available

Notes

See also, data as provided by Lexicon Genetics, Inc.

References

• Original: J:161922 Tang T, et al., A mouse knockout library for secreted and transmembrane proteins. Nat Biotechnol. 2010 Jul;28(7):749-55
• All: 3 reference(s)