C9orf72^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: C9orf72^tm1(KOMP)Mbp
• Name: C9orf72, member of C9orf72-SMCR8 complex; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4950129
• Gene: C9orf72 Location: Chr4:35191285-35226153 bp, - strand Genetic Position: Chr4, 17.13 cM
• Alliance: C9orf72^tm1(KOMP)Mbp page
• IMPC: C9orf72 gene page

Mutation origin

• Mutant Cell Lines: DEPD00552_3_A01, DEPD00552_3_B01, DEPD00552_3_C03, DEPD00552_3_C04, DEPD00552_3_D03, DEPD00552_3_E02, DEPD00552_3_F02
• Germline Transmission: Earliest citation of germline transmission: J:232498
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 7755 starting at position 35211249 and ending at position 35211249 of Chromosome 4 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any C9orf72 Mutation: 36 strains or lines available

Notes

Cell line DEPD00552_3_B01 was successfully used to make chimeric mice. Germline transmission was accomplished. J:232498

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 4 reference(s)