Ldb3^tm6.2Chen
Targeted Allele Detail

Summary

• Symbol: Ldb3^tm6.2Chen
• Name: LIM domain binding 3; targeted mutation 6.2, Ju Chen
• MGI ID: MGI:4949672
• Synonyms: CypherL^-
• Gene: Ldb3 Location: Chr14:34248560-34310639 bp, - strand Genetic Position: Chr14, 20.83 cM, cytoband B
• Alliance: Ldb3^tm6.2Chen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170494
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 12. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 12. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed exon 12, knocking out the long splice variant. The absence of the long form and expression of the short form was confirmed by western blot analysis. (J:170494)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ldb3 Mutation: 46 strains or lines available

References

• Original: J:170494 Cheng H, et al., Selective deletion of long but not short Cypher isoforms leads to late-onset dilated cardiomyopathy. Hum Mol Genet. 2011 May 1;20(9):1751-62
• All: 3 reference(s)