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Et(icre/ERT2)14374Rdav
Transgene Detail
Nomenclature
Symbol: Et(icre/ERT2)14374Rdav
Name: enhancer trap 14374, Ron Davis
MGI ID: MGI:4947570
Transgene: Et(icre/ERT2)14374Rdav  Location: unknown  
Transgene
origin
Strain of Origin:  FVB/N
Project Collection: Neuroscience Blueprint cre
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsAn enhancer trap lentiviral transgenic approach was used to generate these mice. The transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a human synapsin minimal promoter (-244 to +47), a beta-globin intron, an icre /ERT2 fusion (icre; improved with mammalian codon usage, no putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), fused to a mutated human estrogen receptorligand binding domain (ERT2), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability. This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sortm1Sor mice to assess cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to create the 14374 line. (J:150856)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Et(icre/ERT2)14374Rdav     Summary of all recombinase alleles driven by Et(icre/ERT2)14374Rdav.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory