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Et(cre/ERT2)13866Rdav
Transgene Detail
Nomenclature
Symbol: Et(cre/ERT2)13866Rdav
Name: enhancer trap 13866, Ron Davis
MGI ID: MGI:4947562
Transgene: Et(cre/ERT2)13866Rdav  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6J
Project Collection: Neuroscience Blueprint cre
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsAn enhancer trap lentiviral transgenic approach was used to generate these mice. The transgene was designed with a self-inactivating lentiviral vector backbone (FUGW), a human synapsin minimal promoter (-244 to +47), a beta-globin intron, a cre/ERT2 fusion gene (cre fused to a mutated human estrogen receptor ligand binding domain), and a bovine growth hormone polyA signal, all antisense orientation relative to the lentiviral transcript. This packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sortm1Sor mice to assess cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to create the 13866 line. (J:150856)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Et(cre/ERT2)13866Rdav     Summary of all recombinase alleles driven by Et(cre/ERT2)13866Rdav.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory