Et(EGFP/cre)16055Rdav
Transgene Detail
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Symbol: |
Et(EGFP/cre)16055Rdav |
Name: |
enhancer trap 16055, Ron Davis |
MGI ID: |
MGI:4947516 |
Transgene: |
Et(EGFP/cre)16055Rdav Location: unknown
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Alliance: |
Et(EGFP/cre)16055Rdav page
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Strain of Origin: |
C57BL/6J
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Project Collection: |
Neuroscience Blueprint cre
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Transgene Type: |
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Transgenic (Recombinase) |
Mutation: |
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Insertion
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Et(EGFP/cre)16055Rdav expression driven by
1 gene
Transgene expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Et(EGFP/cre)16055Rdav (MGI:4947580) |
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Mutation details: An enhancer trap lentiviral transgenic approach was used to generate these mice. A lentiviral vector transgene construct contained an EGFP/cre fusion gene under control of the mouse Thy1.2 minimal promoter (-270 to +30). The vector was designed with a self-inactivating lentiviral backbone (FUGW). The packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J embryos. Founder mice from line 16055 were crossed to B6-Gt(ROSA)26Sortm1Sor animals to assess cre activity/transmission. Cre positive mice were subsequently bred to C57BL/6 mice to generate a cre positive, reporter negative line.
(J:150856)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Et(EGFP/cre)16055Rdav
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009; |
All: |
2 reference(s) |
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