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Braftm1Bbd
Targeted Allele Detail
Nomenclature
Symbol: Braftm1Bbd
Name: Braf transforming gene; targeted mutation 1, Mariano Barbacid
MGI ID: MGI:4946645
Synonyms: B-RafLSLV600E
Gene: Braf  Location: Chr6:39603237-39725463 bp, - strand  Genetic Position: Chr6, 18.43 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:170095
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready, Hypomorph, Modified isoform(s))
Mutation:    Insertion
 
Mutation detailsAn FRT site and a floxed pgk-hygromycin stop cassette were inserted in intron 14, a GTG to GAG mutation was created in codon 600 and an FRT site was inserted into intron 15 via homologous recombination. RT-PCR analysis indicates the mutant allele is hypomorphic (expressed at about 5-10% of the level of the wild-type allele.) (J:170095)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Braf Mutation:  20 strains or lines available
References
Original:  J:170095 Urosevic J, et al., Constitutive activation of B-Raf in the mouse germ line provides a model for human cardio-facio-cutaneous syndrome. Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):5015-20
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
05/12/2015
MGI 5.21
The Jackson Laboratory