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Tg(BEST1-cre)1Jdun
Transgene Detail
Nomenclature
Symbol: Tg(BEST1-cre)1Jdun
Name: transgene insertion 1, Joshua L Dunaief
MGI ID: MGI:4946605
Synonyms: BEST1-cre
Transgene: Tg(BEST1-cre)1Jdun  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutations:    Insertion, Intergenic deletion
 
Mutation detailsA fragment of the human BEST1 promoter (nucleotides -585 to +38) drives cre recombinase cDNA, the SV40 t-antigen intron, and HSV-TK polyA in the construct. This was purified and microinjected into C57BL/6 zygotes. Six founders were generated with one F2 line that showed the highest percentage of cre immunoreactivity in the RPE was selected for further study. Expression of cre was mosaic, with 50-90% of RPE cells being labeled. This line has a transgene copy number of 20 determined by qPCR analysis (J:170522) or 5 copies (J:240920) inserted about 58 kb upstream of Tmem108 (chr9:103,722,073; NCBI37/mm9) with a 120 bp deletion at the transgene integration site. (J:170522, J:240920)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: BEST1     Summary of all recombinase alleles driven by BEST1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:170522 Iacovelli J, et al., Generation of Cre Transgenic Mice with Postnatal RPE-Specific Ocular Expression. Invest Ophthalmol Vis Sci. 2011 Mar;52(3):1378-83
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
12/05/2017
MGI 6.11
The Jackson Laboratory