Tg(Wnt1-lacZ)R170Rth
Transgene Detail

Summary

• Symbol: Tg(Wnt1-lacZ)R170Rth
• Name: transgene insertion R170, David H Rowitch
• MGI ID: MGI:4946101
• Synonyms: R170
• Transgene: Tg(Wnt1-lacZ)R170Rth Location: unknown
• Alliance: Tg(Wnt1-lacZ)R170Rth page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:69326
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: In this transgene construct, the Wnt1 promoter drives lacZ cDNA, which is followed by the Wnt1 enhancer. Between the promoter and lacZ sequence is a loxP-flanked PGKneo cassette. This stop cassette prevents expression of lacZ in the absence of cre-mediated recombination. Two lines were obtained. R170 has 2 copies of the transgene, while R44 has 3 copies of the reporter. Both lines displayed the expected Wnt1 pattern after E11.5 when crossed to reporter animals. The R170 line showed stronger recombination activity and was used for further studies. (J:69326)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:69326 Danielian PS, et al., Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase. Curr Biol. 1998 Dec 3;8(24):1323-6
• All: 1 reference(s)