Uap1tm1a(KOMP)Mbp
Targeted Allele Detail
|
Symbol: |
Uap1tm1a(KOMP)Mbp |
Name: |
UDP-N-acetylglucosamine pyrophosphorylase 1; targeted mutation 1a, Mouse Biology Program, UC Davis |
MGI ID: |
MGI:4944298 |
Gene: |
Uap1 Location: Chr1:169969507-170002526 bp, - strand Genetic Position: Chr1, 76.84 cM
|
IMPC: |
Uap1 gene page |
|
Mutant Cell Lines: |
DEPD0007_2_A09, DEPD0007_2_A11, DEPD0007_2_A12, DEPD0007_2_B09, DEPD0007_2_B10, DEPD0007_2_B11, DEPD0007_2_C10, DEPD0007_2_C11, DEPD0007_2_C12, DEPD0007_2_D09, DEPD0007_2_D10, DEPD0007_2_D12, DEPD0007_2_E10, DEPD0007_2_E11, DEPD0007_2_E12, DEPD0007_2_F10, DEPD0007_2_F11, DEPD0007_2_F12, DEPD0007_2_G09, DEPD0007_2_G12, DEPD0007_2_H10 |
Germline Transmission: |
Unknown
|
Parent Cell Line: |
JM8A1.N3 (ES Cell)
|
Strain of Origin: |
C57BL/6N-Atm1Brd
|
Project Collection: |
KOMP-CSD
|
|
Allele Type: |
|
Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
|
Insertion
Vector: L1L2_Bact_P
|
|
|
Mutation details: The L1L2_Bact_P cassette was inserted at position 169988655 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 169989576. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157064)
|
|
|
|
Original: |
J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010; |
All: |
1 reference(s) |
|