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Rasal2tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Rasal2tm1a(EUCOMM)Wtsi
Name: RAS protein activator like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4939768
Gene: Rasal2  Location: Chr1:157135182-157412595 bp, - strand  Genetic Position: Chr1, 67.97 cM
Mutation
origin
Mutant Cell Lines:  EPD0732_1_A03, EPD0732_1_A05, EPD0732_1_A09, EPD0732_1_A12, EPD0732_1_B02, EPD0732_1_B03, EPD0732_1_B04, EPD0732_1_B09, EPD0732_1_B12, EPD0732_1_C01, EPD0732_1_C03, EPD0732_1_C05, EPD0732_1_C09, EPD0732_1_C10, EPD0732_1_C12, EPD0732_1_D01, EPD0732_1_D08, EPD0732_1_D10, EPD0732_1_D11, EPD0732_1_E03, EPD0732_1_E08, EPD0732_1_E09, EPD0732_1_E10, EPD0732_1_F02, EPD0732_1_F04, EPD0732_1_F12, EPD0732_1_G01, EPD0732_1_G04, EPD0732_1_G05, EPD0732_1_H01, EPD0732_1_H03, EPD0732_1_H05, EPD0732_1_H07, EPD0732_1_H08, EPD0732_1_H09, EPD0732_1_H11
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 157193316 of Chromosome 1 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 157192507. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rasal2 Mutation:  41 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
05/15/2018
MGI 6.12
The Jackson Laboratory