Tg(Mlana-cre)5Bee
Transgene Detail

Summary

• Symbol: Tg(Mlana-cre)5Bee
• Name: transgene insertion 5, Friedrich Beermann
• MGI ID: MGI:4887843
• Synonyms: MART1-cre, MART-1::Cre
• Transgene: Tg(Mlana-cre)5Bee Location: unknown
• Alliance: Tg(Mlana-cre)5Bee page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Mlana-cre)5Bee expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mlana (MGI:108454)

• Mutation details: BAC clone RP23-308B16 was modified by recombineering methods so that a cre-polyA cassette with an FRT-ampicillin-FRT selection cassette replaced the start codon of Mlana (MART-1). Cre expression is controlled by the Mlana (MART-1) regulatory sequences. The targeting construct was electorporated into bacterial cells for homologous recombination and selection, followed by induction of Flp-mediated excision of the ampicillin cassette. The BAC construct was injected into fertilized oocytes and 5 founder lines, 4 of which showed reporter expression in pigment cells when crossed to ROSA26-R mice. Line 5 was propagated for futher analysis. (J:172135)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mlana (mouse) Summary of all recombinase alleles driven by Mlana. Alliance mouse Mlana gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:172135 Aydin IT, et al., A mart-1::Cre transgenic line induces recombination in melanocytes and retinal pigment epithelium. Genesis. 2011 May;49(5):403-9
• All: 5 reference(s)