Tg(Pmch-cre)1Lowl
Transgene Detail
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Symbol: |
Tg(Pmch-cre)1Lowl |
Name: |
transgene insertion 1, Bradford B Lowell |
MGI ID: |
MGI:4887235 |
Synonyms: |
MCH-Cre |
Transgene: |
Tg(Pmch-cre)1Lowl Location: unknown
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Alliance: |
Tg(Pmch-cre)1Lowl page
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Transgene Type: |
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Transgenic (Recombinase) |
Mutation: |
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Insertion
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Tg(Pmch-cre)1Lowl expression driven by
1 gene
Transgene expression driven by:
Organism |
Driver Gene |
Note |
Not Specified |
Pmch |
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Mutation details: Homologous recombination with EL250 cells was induced to insert a cre-polyA-FRT-Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further.
(J:167906)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Pmch
(species not specified)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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Original: |
J:167906 Kong D, et al., Glucose stimulation of hypothalamic MCH neurons involves K(ATP) channels, is modulated by UCP2, and regulates peripheral glucose homeostasis. Cell Metab. 2010 Nov 3;12(5):545-52 |
All: |
28 reference(s) |
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