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Kdm1atm1(NCOM)Mfgc
Targeted Allele Detail
Nomenclature
Symbol: Kdm1atm1(NCOM)Mfgc
Name: lysine (K)-specific demethylase 1A; targeted mutation 1, Mammalian Functional Genomics Centre
MGI ID: MGI:4880311
Gene: Kdm1a  Location: Chr4:136550540-136602723 bp, - strand  Genetic Position: Chr4, 68.8 cM
Mutation
origin
Mutant Cell Lines:  N01650P1_C_143W_E7, N01650P1_C_143W_E11, N01650P1_C_143W_F9
Germline Transmission:  Earliest citation of germline transmission: J:211773
Parent Cell Line:  C2 (Nagy) (ES Cell)
Strain of Origin:  C57BL/6NCrl
Project Collection: NorCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_GOHANU
 
Mutation detailsThe insertion of NorCOMM cassette pGOHANU created a deletion of size 928bp between positions 136149570-136148642 of Chromosome 4 (Genome Build NCBIM37). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165964)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Kdm1a Mutation:  29 strains or lines available
References
Original:  J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/13/2017
MGI 6.10
The Jackson Laboratory