About   Help   FAQ
Targeted Allele Detail
Symbol: Itpr3tm1(NCOM)Mfgc
Name: inositol 1,4,5-triphosphate receptor 3; targeted mutation 1, Mammalian Functional Genomics Centre
MGI ID: MGI:4880235
Gene: Itpr3  Location: Chr17:27057304-27122223 bp, + strand  Genetic Position: Chr17, 13.68 cM
Mutant Cell Lines:  N01293P1_C_239W_B6, N01293P1_C_239W_C5, N01293P1_C_239W_C6, N01293P1_C_239W_D6, N01293P1_C_239W_E5, N01293P1_C_239W_F5, N01293P1_C_239W_F6, N01293P1_C_239W_H5
Germline Transmission:  Earliest citation of germline transmission: J:201643
Parent Cell Line:  C2 (Nagy) (ES Cell)
Strain of Origin:  C57BL/6NCrl
Project Collection: NorCOMM
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_GOHANU
Mutation detailsThe insertion of NorCOMM cassette pGOHANU created a deletion of size 1030bp between positions 27209573-27210603 of Chromosome 17 (Genome Build NCBIM37). This deletion results in the removal of functionally critical exon 2. The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165964)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Itpr3 Mutation:  113 strains or lines available
Cell line N01293P1_C_239W_C5 was successfully used to make chimeric mice. Germline transmission was accomplished. J:201643
Original:  J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.16
The Jackson Laboratory