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Transgene Detail
Symbol: Tg(ACTB-tTA2,-MAPT/lacZ)1Luo
Name: transgene insertion 1, Liqun Luo
MGI ID: MGI:4867698
Synonyms: CAG-stop-tTA2, CAG-stop-tTA2-IRES-tau-lacZ, ii-CAG-stop-tTA2-IRES-tau-lacZ-ii, Tg(ACTB-tTA2,-lacZ)1Luo
Transgene: Tg(ACTB-tTA2,-MAPT/lacZ)1Luo  Location: unknown  
Strain of Origin:  DBA/2
Transgene Type:    Transgenic (Reporter, Transactivator)
Mutation:    Insertion
Mutation detailsThe ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene was designed with the human cytomegalovirus (CMV) enhancer/chicken beta-actin core promoter (pCA), a loxP-flanked neomycin resistance gene (neor) with transcriptional STOP cassette, a modified tetracycline-regulated transactivator gene (tTA2), an internal ribosome entry site (IRES), and a bovine microtubule-associated protein tau with beta-galactosidase fusion protein (tau-lacZ). The transgene was further modified to have two copies of a ~250 bp chicken beta-globin HS4 insulator sequence (separated by an ApaLI restriction site) on each side of the transgene. The 5' insulator pair is flanked by Saw1 and Kpn1 restriction sites, while the 3' insulator pair is flanked by Spe1 and Asc1 restriction sites. (J:167275)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:167275 Miyamichi K, et al., Cortical representations of olfactory input by trans-synaptic tracing. Nature. 2011 Apr 14;472(7342):191-6
All:  4 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory