Tg(ACTB-tTA2,-MAPT/lacZ)1Luo
Transgene Detail

Summary

• Symbol: Tg(ACTB-tTA2,-MAPT/lacZ)1Luo
• Name: transgene insertion 1, Liqun Luo
• MGI ID: MGI:4867698
• Synonyms: CAG-stop-tTA2, CAG-stop-tTA2-IRES-tau-lacZ, ii-CAG-stop-tTA2-IRES-tau-lacZ-ii, Tg(ACTB-tTA2,-lacZ)1Luo
• Transgene: Tg(ACTB-tTA2,-MAPT/lacZ)1Luo Location: unknown
• Alliance: Tg(ACTB-tTA2,-MAPT/lacZ)1Luo page

Transgene origin

• Strain of Origin: DBA/2

Transgene description

• Transgene Type: Transgenic (Reporter, Transactivator)
• Mutation: Insertion
• Mutation details: The ii-CAG-stop-tTA2-IRES-tau-lacZ-ii transgene was designed with the human cytomegalovirus (CMV) enhancer/chicken beta-actin core promoter (pCA), a loxP-flanked neomycin resistance gene (neor) with transcriptional STOP cassette, a modified tetracycline-regulated transactivator gene (tTA2), an internal ribosome entry site (IRES), and a bovine microtubule-associated protein tau with beta-galactosidase fusion protein (tau-lacZ). The transgene was further modified to have two copies of a ~250 bp chicken beta-globin HS4 insulator sequence (separated by an ApaLI restriction site) on each side of the transgene. The 5' insulator pair is flanked by Saw1 and Kpn1 restriction sites, while the 3' insulator pair is flanked by Spe1 and Asc1 restriction sites. (J:167275)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:167275 Miyamichi K, et al., Cortical representations of olfactory input by trans-synaptic tracing. Nature. 2011 Apr 14;472(7342):191-6
• All: 5 reference(s)