Mcm5tm1a(KOMP)Mbp
Targeted Allele Detail
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Symbol: |
Mcm5tm1a(KOMP)Mbp |
Name: |
minichromosome maintenance complex component 5; targeted mutation 1a, Mouse Biology Program, UC Davis |
MGI ID: |
MGI:4843726 |
Gene: |
Mcm5 Location: Chr8:75836197-75855067 bp, + strand Genetic Position: Chr8, 35.59 cM
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IMPC: |
Mcm5 gene page |
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Mutant Cell Lines: |
DEPD0002_11_A08, DEPD0002_11_B08, DEPD0002_11_C07, DEPD0002_11_D08, DEPD0002_11_E10, DEPD0002_11_E12, DEPD0002_11_F07, DEPD0002_11_F09, DEPD0002_11_F10, DEPD0002_11_H09, DEPD0002_11_H11, DEPD0002_11_H12 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A1.N3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 75845441 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 75848594. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157064, J:274368)
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Cell line DEPD0002_11_F10 was successfully used to make chimeric mice. Germline transmission was/was not accomplished. J:274368
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Original: |
J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010; |
All: |
2 reference(s) |
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