Hprt1^{tm1(sb-Onco-Array)Peli}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(sb-Onco-Array)Peli}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Pentao Liu
• MGI ID: MGI:4843327
• Synonyms: Hprt-OncoArray, Onc, PLND
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(sb-Onco-Array)Peli} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166283
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (RMCE-ready, Transposon concatemer)
• Mutation: Insertion
• Mutation details: Using recombination mediated cassette exchange (RMCE) a modified BAC containing 9 serially ligated transposons was introduced into the RMCE ready locus Hprt^tm1Brd. The modified BAC vector also included a loxP site, a neo cassette, FRT sites flanking the serially ligated transposons, 2 poly A signals and a lox511 site. Each transposon consisted of sleeping beauty inverted terminal repeats flanking a splice acceptor site, a variable cDNA, and a poly A signal. The cDNAs used in each transposon were, in order from 5' to 3'; a human MYC cDNA, a human KRAS2B isoform specific cDNA containing a G12D mutation, a human KRAS2A isoform specific cDNA containing a G12D mutation, GFP, lacZ, a human BRAF cDNA, a human KRAS2A isoform specific cDNA, a human BRAF cDNA containing a V600E mutation, and a human KRAS2B isoform specific cDNA. (J:166283)

Tumor Data

List all tumor models in MMHCdb carrying Hprt1^{tm1(sb-Onco-Array)Peli}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

References

• Original: J:166283 Su Q, et al., A DNA transposon-based approach to validate oncogenic mutations in the mouse. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19904-9
• All: 1 reference(s)