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Fbxo7tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Fbxo7tm1a(EUCOMM)Wtsi
Name: F-box protein 7; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4842259
Gene: Fbxo7  Location: Chr10:86021972-86051873 bp, + strand  Genetic Position: Chr10, 41.91 cM
Mutation
origin
Mutant Cell Lines:  EPD0622_3_A01, EPD0622_3_A04, EPD0622_3_A05, EPD0622_3_B02, EPD0622_3_B03, EPD0622_3_B04, EPD0622_3_B05, EPD0622_3_B06, EPD0622_3_C03, EPD0622_3_C05, EPD0622_3_D01, EPD0622_3_D02, EPD0622_3_D04, EPD0622_3_E02, EPD0622_3_E03, EPD0622_3_F03, EPD0622_3_F04, EPD0622_3_F05, EPD0622_3_G01, EPD0622_3_G02, EPD0622_3_G03, EPD0622_3_G06, EPD0622_3_H01, EPD0622_3_H05
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 86030441 of Chromosome 10 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 86031289. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Fbxo7 Mutation:  7 strains or lines available
Notes
Special note about mutant cell line EPD0622_3_D02: The mutation generated in this ES cell line results in a hypomorphic allele with 4-16% residual Fbxo7 transcript depending on the tissue tested, indicating incomplete ablation of gene expression (J:272313).
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/10/2020
MGI 6.16
The Jackson Laboratory