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Daam2tm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Daam2tm1a(KOMP)Wtsi
Name: dishevelled associated activator of morphogenesis 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4841108
Gene: Daam2  Location: Chr17:49456022-49564343 bp, - strand  Genetic Position: Chr17, 25.5 cM
Mutation
origin
Mutant Cell Lines:  EPD0660_3_A09, EPD0660_3_B10, EPD0660_3_B12, EPD0660_3_C12, EPD0660_3_E09, EPD0660_3_E10, EPD0660_3_E11, EPD0660_3_F09, EPD0660_3_F10, EPD0660_3_F11, EPD0660_3_G10, EPD0660_3_H09, EPD0660_3_H10, EPD0660_3_H11
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Pgk_P
 
Mutation detailsThe L1L2_Pgk_P cassette was inserted at position 49490811 of Chromosome 17 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 49489731. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Daam2 Mutation:  1 strain or line available
Notes
Cell line EPD0660_3_A09 was successfully used to make chimeric mice. Germline transmission was accomplished. J:204986
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/26/2020
MGI 6.15
The Jackson Laboratory