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Targeted Allele Detail
Symbol: Tbc1d2tm1(NCOM)Cmhd
Name: TBC1 domain family, member 2; targeted mutation 1, Centre for Modelling Human Disease
MGI ID: MGI:4839135
Gene: Tbc1d2  Location: Chr4:46604390-46650209 bp, - strand  Genetic Position: Chr4, 25.04 cM
Mutant Cell Lines:  N00020P0_C_5T_A11, N00020P0_C_5T_B10, N00020P0_C_5T_B12, N00020P0_C_5T_C9, N00020P0_C_5T_C11, N00020P0_C_5T_C12, N00020P0_C_5T_D11, N00020P0_C_5T_E11
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  C2 (Nagy) (ES Cell)
Strain of Origin:  C57BL/6NCrl
Project Collection: NorCOMM
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_NTARU-0
Mutation detailsThe insertion of NorCOMM cassette pNTARU created a deletion of size 381bp between positions 46659269-46658888 of Chromosome 4 (Genome Build NCBIM37). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165963)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Tbc1d2 Mutation:  14 strains or lines available
Original:  J:165963 Centre for Modeling Human Disease, Alleles produced for the NorCOMM project by the Centre for Modeling Human Disease (Cmhd), Institute of Biomaterials & Biomedical Engineering, University of Toronto. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
The Jackson Laboratory