About   Help   FAQ
Arap1tm1(NCOM)Cmhd
Targeted Allele Detail
Nomenclature
Symbol: Arap1tm1(NCOM)Cmhd
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1; targeted mutation 1, Centre for Modelling Human Disease
MGI ID: MGI:4839133
Gene: Arap1  Location: Chr7:101348067-101412586 bp, + strand  Genetic Position: Chr7, 54.56 cM, cytoband F1
Mutation
origin
Mutant Cell Lines:  N00016P0_C_5T_A5, N00016P0_C_5T_A6, N00016P0_C_5T_D5, N00016P0_C_5T_D6, N00016P0_C_5T_F5, N00016P0_C_5T_H5, N00016P0_C_5T_H7 (CMHD)
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  C2 (Nagy) (ES Cell)
Strain of Origin:  C57BL/6NCrl
Project Collection: NorCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_NTARU-0
 
Mutation detailsThe insertion of NorCOMM cassette pNTARU created a deletion of size 337bp between positions 108546709-108547046 of Chromosome 7 (Genome Build NCBIM37). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165963)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Arap1 Mutation:  37 strains or lines available
References
Original:  J:165963 Centre for Modeling Human Disease, Alleles produced for the NorCOMM project by the Centre for Modeling Human Disease (Cmhd), Institute of Biomaterials & Biomedical Engineering, University of Toronto. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
03/21/2017
MGI 6.08
The Jackson Laboratory