Epn3^tm1.1Pdc
Targeted Allele Detail

Summary

• Symbol: Epn3^tm1.1Pdc
• Name: epsin 3; targeted mutation 1.1, Pietro De Camilli
• MGI ID: MGI:4838863
• Gene: Epn3 Location: Chr11:94380425-94390800 bp, - strand Genetic Position: Chr11, 58.9 cM, cytoband C
• Alliance: Epn3^tm1.1Pdc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167134
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J x 129S1/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert a loxP site upstream of the initiation site of the Epsin 3 (Epn3) gene. A frt-flanked neomycin resistance (neo) cassette, followed by a second loxP site, was placed downstream of exon 9. Cre mediated recombination deleted exons 1 through 9 and the neo. The absence of protein expression was confirmed by immunohistochemistry on parietal cells of the gastric glands. (J:167134)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Epn3 Mutation: 30 strains or lines available

References

• Original: J:167134 Ko G, et al., Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi. Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21511-6
• All: 2 reference(s)