Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak}
Transposon induced Allele Detail

Summary

• Symbol: Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak}
• Name: diacylglycerol kinase, beta; transposon insertion IR3.PV122, Junji Takeda
• MGI ID: MGI:4836984
• Synonyms: Dgkb^{Tn(sb-lacZ,GFP)IR3.1Hhar}, Dgkb^{Tn(sb-lacZ,GFP)IR3.1Jtak}
• Gene: Dgkb Location: Chr12:37930169-38684238 bp, + strand Genetic Position: Chr12, 17.11 cM
• Alliance: Dgkb^{Tn(sb-lacZ,GFP)IR3.PV122Jtak} page

Mutation origin

• Strain of Origin: mixed

Mutation description

• Allele Type: Transposon induced (Reporter)
• Mutations: Intragenic deletion, Transposon insertion
• Mutation details: Transposition of a single copy of a sleeping beauty transposable element gene trap vector occurred between the 22nd and 23rd exons among 25 exons of this gene. This gene trap vector consists of the inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking human BCL2 splice acceptor sequence, IRES, lacZ, poly A signal, a chimeric CAG promoter containing sequence from the chicken ACTB promoter, GFP, and splice donor sequence. This allele was generated from mice carrying TgTn(sb-lacZ,GFP)IR3Jtak and Tg(CAG-sb10)1Jtak. Western blotting with an N-terminal binging antibody revealed that there was no expression in the mouse, as was confirmed by immunohistochemistry of hippocampus, cerebral cortex, and caudate putamen. (J:104039, J:104043)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dgkb Mutation: 52 strains or lines available

References

• Original: J:104043 Keng VW, et al., Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system. Nat Methods. 2005 Oct;2(10):763-9
• All: 6 reference(s)