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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Hans Clevers
MGI ID: MGI:4835542
Synonyms: R26R-Brainbow2.1, R26R-Confetti
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Germline Transmission:  Earliest citation of germline transmission: J:164644
Parent Cell Line:  IB10/E14IB10 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
Mutation detailsA targeting vector containing (from 5' to 3') a strong CAGG promoter, a loxP site, a PGK-Neo-pA cassette (serving as a transcriptional roadblock), and the Brainbow 2.1 construct. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The Brainbow 2.1 construct was designed by Drs. Jeff Lichtman and Joshua Sanes (Harvard University) with four fluorescent protein sequences uniquely positioned in a tandem fashion and delimited by loxP sites in opposite orientation. Specifically, this Brainbow 2.1 coding region is composed of two adjacent floxed head-to-tail tandem dimers. The first head-to-tail dimer contains a loxP site and humanized Renilla GFP (hrGFPII; with nuclear localization signal plus polyA sequence) in forward orientation and a loxP site and monomeric EYFP (mYFPA206K plus polyA sequence) in reverse orientation. The second head-to-tail dimer contains a loxP site and tdimer2(12) RFP plus polyA sequence in forward orientation, and a loxP site and mCerulean CFP (with membrane tethering palmitoylation sequence plus polyA sequence) in reverse orientation. A single frt site is located at the 3' end of the Brainbow 2.1 construct. (J:125961, J:164644)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  497 strains or lines available
Original:  J:164644 Snippert HJ, et al., Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Cell. 2010 Oct 1;143(1):134-44
All:  112 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory